Immunological detection of hymenopteran parasitism in Helicoverpa zea and Heliothis virescens
Document Type
Article
Publication Title
Biological Control
Abstract
We have developed a monoclonal antibody that recognizes the larval and adult stages of Microplitis croceipes (Cresson) (Hymenoptera: Braconidae) and Cotesia marginiventris (Cresson) (Hymenoptera: Braconidae), two principal endoparasitoids of the polyphagous pests Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) and Heliothis virescens (F.) (Lepidoptera: Noctuidae). The antibody has been incorporated into an indirect enzyme-linked immunosorbent assay that can be used to distinguish parasitized from unparasitized pests. The antibody cross-reacts with several different hymenopteran parasitoids, but not with any of the noctuid pests we have assayed. An immunoassay based on a broadly reactive antibody such as this one enables comprehensive detection of hymenopteran parasitism with a single antibody reagent.
First Page
197
Last Page
202
DOI
10.1006/bcon.1997.0514
Publication Date
1-1-1997
Recommended Citation
Stuart, Melissa K. and Greenstone, Matthew H., "Immunological detection of hymenopteran parasitism in Helicoverpa zea and Heliothis virescens" (1997). All KCOM Faculty Publications. 335.
https://scholarworks.atsu.edu/kcom-faculty/335