Microvascular injury after ischemia and reperfusion in skeletal muscle of exercise-trained rats

Document Type

Article

Publication Title

Journal of Applied Physiology

Abstract

Ischemia and reperfusion in skeletal muscle is associated with increases in total vascular resistance (Rt) and the microvascular permeability to plasma proteins. To determine whether exercise training can attenuate ischemia and reperfusion-induced microvascular injury in skeletal muscle, intact (with skin) and skinned, maximally vasodilated (papaverine), isolated hindquarters of control (C) and exercise-trained (ET) rats were subjected to ischemia (intact 130 min; skinned 60 min) followed by 60 min of reperfusion. ET rats ran on a motorized treadmill at 32 m/min (8% grade), 2 h/day for 12 wk, whereas the C rats were cage confined. Before ischemia, ET hindquarters had higher isogravimetric flow, lower Rt, and similar solvent drag reflection coefficients (σ(f)) compared with C. During reperfusion in intact hindquarters, flow was higher (P < 0.05) and Rt tended to be lower (15 ± 2 vs. 25 ± 5 mmHg · ml-1 · min · 100 g; P < 0.1) in ET compared with C; however, in skinned hindquarters flow and Rt (14 ± 2 vs. 13 ± 2 mmHg · ml-1 · min · 100 g) were not different between C and ET. During reperfusion, σ(f) was reduced (P < 0.05) in both intact (C 0.68 ± 0.03; ET 0.68 ± 0.02) and skinned (C 0.66 ± 0.03; ET 0.68 ± 0.03) hindquarters, indicative of an increased microvascular permeability to plasma proteins. These results indicate that exercise training did not attenuate the microvascular injury (increased Rt and decreased σ(f)) associated with ischemia and reperfusion in rat skeletal muscle.

First Page

2329

Last Page

2336

DOI

10.1152/jappl.1990.68.6.2329

Publication Date

1-1-1990

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