Cholesterylene, a newly recognized tissue lipid, found at high levels in the cornea

Document Type

Article

Publication Title

Biochemical and Biophysical Research Communications

Abstract

In the course of measuring the concentration of cholesterol in an opacified dog cornea by gas-chromatography, relatively large amounts of an unidentified non-saponifiable lipid were recognized. When the unknown lipid was subjected to gas chromatographic-mass spectral analysis it displayed a major ion at m/z 368 M+ and was identified as cholesta-3,5-diene, cholesterylene, by computer match with mass spectral-registry data. Cholesterylene was then shown to be present in the corneas of normal dogs, cows and humans, accounting for 20-25% of the total steroid-sterol in dog corneas and 5-10% in cow and human. Cholesterylene, which can be considered as an extremely nonpolar dehydration product of cholesterol, has not previously been recognized in animal tissues. Although the source of corneal cholesterylene is unknown, preliminary results suggest non-enzymatic formation from cholesterol. © 1992 Academic Press, Inc.

First Page

1647

Last Page

1655

DOI

10.1016/S0006-291X(05)81597-9

Publication Date

8-14-1992

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