Diethylstilbestrol increases intracellular calcium in lens epithelial cells

Document Type

Article

Publication Title

Pflugers Archiv European Journal of Physiology

Abstract

The effects of diethylstilbestrol (DES) on steady-state intracellular calcium concentration ([Ca2+]i) and resting Ca 2+ influx were examined in primary cultures of bovine lens epithelial cells using conventional fluorometric techniques (Fura-2). At low concentrations (10 μM), DES usually induced relatively rapid increases in [Ca2+]i that occurred over an interval of 10-50 s and that persisted for several minutes in the continued presence of the drug. In about 10% of the cells, cyclic oscillations in [Ca2+]i were seen after adding 10 μM DES. At higher concentrations (100 μM), the drug induced more prolonged increases in [Ca2+]i lasting several minutes. DES did not affect Mn2+ quench determinations of resting Ca2+ influx, and neither 100 μM GdCl3, which blocked resting Ca2+ influx, nor low [Ca2+]o solutions substantially diminished the influence of DES on [Ca 2+]i. Pretreatment of cells with the smooth endoplasmic reticulum Ca2+ ATPase (SERCA) inhibitors cyclopiazonic acid (CPA) or thapsigargin completely abolished the effect of 10 μM DES on [Ca 2+]i, while the IP3 receptor blocker 2-aminoethoxydiphenyl borane (2-APB) had no effect. These results indicate that DES releases CPA-sensitive stores of intracellular Ca2+, perhaps by inhibiting SERCA-dependent Ca2+ sequestration. © Springer-Verlag 2005.

First Page

145

Last Page

154

DOI

10.1007/s00424-005-1398-2

Publication Date

6-1-2005

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