Critical-size defects reconstruction with four different bone grafts associated with e-PTFE membrane: A histomorphometric experimental in vivo study

Authors

Gustavo Vicentis Oliveira Fernandes, Periodontics and Oral Medicine Department, University of Michigan School of Dentistry, Ann Arbor, MI, USA.
Filipe Castro, Universidade Fernando Pessoa, 4249-004 Porto, Portugal.
Rafael Martins Pereira, Periodontics and Oral Medicine Department, University of Michigan School of Dentistry, Ann Arbor, Michigan, USA.
Wendel Teixeira, Department of Biologic and Materials Sciences & Prosthodontics, University of Michigan School of Dentistry, Ann Arbor, Michigan, USA.
Sergio Gehrke, Department of Research, Bioface/PgO/UCAM, Montevideo, Uruguay.
Julio Cesar Joly, São Leopoldo Mandic, Campinas, São Paulo, Brazil.
Juan Blanco Carrion, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.

Document Type

Article

Abstract

Abstract

Objectives: The goal of this study was to assess the newly formed bone and the remnant biomaterial by comparing four different bone grafts used to treat critical-size defects, associated or not with the non-resorbable membrane.

Materials and methods: Two calvaria critical-size bone defects were created in 50 male Wistar rats. They were divided into blood (G1), autogenous (G2), bioglass (G3), hydroxyapatite (G4), and xenograft (G5) groups, associated or not with e-PTFE. The experimental periods were 15 and 45 days. Sections were prepared for histomorphometric assessment. All data were analyzed by the mixed-effects model with multiple comparisons (significance level, p < .05).

Results: A similar level of new bone was observed for all groups, associated with a high level of vascularization. G1 and G2 ensured sovereignty over the greater quantity of new bone. A non-significant result was reported comparing groups with and without membranes. No significant result was found between the experimental synthetic biomaterials (G3 and G4). G5L achieved 22.0% of new bone after 45 days (p > .05). All groups had a stable volume of biomaterial kept in the short term (p > .05). G2 was the best material for new bone formation and final volume of biomaterial, followed by G4 < G5 < G3. Thus, it is possible that G4 had a better degradation profile among the experimental groups.

Conclusions: The best results were found in the autogenous group, with higher resorption and integration; non-significative new bone was found among the experimental groups; and the regeneration of critical bone defects using an e-PTFE barrier did not present significant results on new bone formation.

First Page

167

Last Page

178

DOI

10.1111/clr.14210

Publication Date

2-2024

Comments

Fernandes, G. V. O., Castro, F., Pereira, R. M., Teixeira, W., Gehrke, S., Joly, J. C., Blanco Carrion, J., & Fernandes, J. C. H. (2024). Critical-size defects reconstruction with four different bone grafts associated with e-PTFE membrane: A histomorphometric experimental in vivo study. Clinical oral implants research, 35(2), 167–178.

https://doi.org/10.1111/clr.14210

Accepted Version

12 Months embargo, Publisher source must be acknowledged with citation, Must link to publisher version with set statement (see policy) and DOI

Recommended Citation

Fernandes, G. V. O., Castro, F., Pereira, R. M., Teixeira, W., Gehrke, S., Joly, J. C., Blanco Carrion, J., & Fernandes, J. C. H. (2024). Critical-size defects reconstruction with four different bone grafts associated with e-PTFE membrane: A histomorphometric experimental in vivo study. Clinical oral implants research, 35(2), 167–178.